Publications
( * equal contribution; † corresponding author )
Rapid biosynthesis of glycoprotein therapeutics and vaccines from freeze-dried bacterial cell lysates
Stark, J. C. †, Jaroentomeechai, T., Warfel, K. F., Hershewe, J. M., DeLisa, M. P. †, & Jewett, M. C. †
Nature Protocols (2023) doi.org/10.1038/s41596-022-00799-z
MYC driven synthesis of Siglec ligands is a novel glyco-immune checkpoint
Smith, B. A. H. *, Deutzmann, A. *, Correa, K. M., Delaveris, C. S., Dhanasekaran, R., Dove, C. G., Sullivan, D. K., Wisnovsky, S., Stark, J. C., Pluvinage, J. V., Swaminathan, S., Riley, N. M., Rajan, A., Majeti, R., Felsher, D. W. †, & Bertozzi, C. R. †
PNAS (2023) doi.org/10.1073/pnas.2215376120
Antibody-lectin chimeras for glyco-immune checkpoint blockade
Stark, J. C. *, Gray, M. A. *, Wisnovsky, S. , Ibarlucea-Benitez, I., Lustig, M., Riley, N. M., Ribi, M. K., Errington, W. J., Bruncsics, B., Sarkar, C. A., Valerius, T., Ravetch, J. V., & Bertozzi, C. R. †
bioRxiv (2022) doi: 10.1101/2022.10.26.513931
Genome-wide, bidirectional CRISPR screens identify mucins as critical host factors modulating SARS-CoV-2 infection
Biering, S. B. *, Sarnik, S. A. *, Wang, E., Zengel, J. R., Leist, S. R., Schäfer, A., Sathyan, V., Hawkins, P., Okuda, K., Tau, C., Jangid, A. R., Duffy, C. R., Wei, J., Gilmore, R. C., Alfajaro, M. M., Strine, M. S., Nguyenla, X., Van Dis, E., Catamura, C., Yamashiro, L. H., Belk, J. A., Begeman, A., Stark, J. C., Shon, D. J., Fox, D. M., Ezzatpour, S., Huang, E., Olegario, N., Rustagi, A., Volmer, A. S., Livraghi-Butrico, A., Wehri, E., Behringer, R. B., Cheon, D., Schaletzky, J., Aguilar, H. C., Puschnik, A. S., Button, B., Pinsky, B. A., Blish, C. A., Baric, R. S., O’Neal, W. K., Bertozzi, C. R., Wilen, C. B., Boucher, R. C., Carette, J. E., Stanley, S. A., Harris, E. †, Konermann, S. †, & Hsu, P. D. †
Nature Genetics (2022) doi: 10.1038/s41588-022-01131-x
Targeting hypersialylation in multiple myeloma represents a novel therapeutic strategy to enhance NK cell-mediated tumor responses
Daly, J., Sarkar, S., Natoni, A., Stark, J. C., Riley N. M., Bertozzi, C. R., Carlsten, M. †, O’Dwyer, M. †
Blood Advances (2022) doi: 10.1182/bloodadvances.2021006805
Implementing hands-on molecular and synthetic biology education using cell-free technology
Huang, A. †, Bryan, B., Kraves, S., Alvarez-Saavedra, E., Stark, J. C. †
Methods in Molecular Biology (2022) doi: 10.1007/978-1-1998-8_25
Synthetic Siglec-9 agonists inhibit neutrophil activation associated with COVID-19
Delaveris, C. S., Wilk, A. J., Riley, N. M., Stark, J. C., Yang, S. S., Rogers, A. J., Ranganath, T., Nadeau, K. C., The Stanford COVID-19 Biobank, Blish, C. A., & Bertozzi, C. R. †
ACS Central Science (2021) doi: 10.1021/acscentsci.0c01669
On-demand biomanufacturing of protective conjugate vaccines
Stark, J. C. *, Jaroentomeechai, T. *, Moeller, T. D., Hershewe, J. M., Warfel, K. F., Moricz, B. S., Martini, A. M., Dubner, R. S., Hsu, K. J., Stevenson, T. C., Jones, B. D., DeLisa, M. P. †, & Jewett, M. C. †
Science Advances (2021) doi: 10.1126/sciadv.abe9444
Featured in Nature, PNAS, preLights, Vice, Northwestern University News, Cornell University News
A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
Kightlinger, W., Duncker, K. E., Ramesh, A., Thames, A. H., Natarajan, A., Stark, J. C., Yang, A., Lin, L., Mrksich, M., DeLisa, M. P., & Jewett, M. C. †
Nature Communications (2019) doi: 10.1038/s41467-019-12024-9
Featured in Northwestern Engineering News
BioBits™ Health: Classroom activities exploring engineering, biology, and human health with fluorescent readouts
Stark, J. C., Huang, A., Hsu, K. J., Dubner, R. S., Forbrook, J., Marshalla, S., Rodriguez, F., Washington, M., Rybnicky, G. A., Nguyen, P. Q., Hasselbacher, B., Jabri, R., Kamran, R., Koralewski, V., Wightkin, W., Martinez, T., & Jewett, M. C. †
ACS Synthetic Biology (2019) doi: 10.1021/acssynbio.8b00381
Featured in Northwestern University News, National Science Foundation News & Blog
BioBits™ Bright: A fluorescent synthetic biology education kit
Stark, J. C. *, Huang, A. *, Nguyen, P. Q. *, Dubner, R. S., Hsu, K. J., Ferrante, T. C., Anderson, M. A., Kanapskyte, A., Mucha, Q., Packett, J. S., Patel, P., Patel, R., Qaq, D., Zondor, T., Burke, J., Martinez, T., Miller-Berry, A., Puppala, A., Reichert, K., Schmid, M., Brand, L., Chellaswamy, J. F., Faheem, N., Fetherling, S., Gong, E., Gonzalzles, E. M., Granito, T., Koritsaris, J., Nguyen, B., Ottman, S., Palffy, C., Patel, A., Skweres, S., Slaton, A., Woods, T., Donghia, N., Pardee, K., Collins, J. J. †, & Jewett, M. C. †
Science Advances (2018) doi: 10.1126/sciadv.aat5107
Featured in Science, NPR, Northwestern Engineering Magazine, Northwestern University News, MIT News, Wyss Institute News, Addgene, The Science Breaker, Army Research News, National Science Foundation News
BioBits™ Explorer: A modular synthetic biology education kit
Huang, A. *, Nguyen, P. Q. *, Stark, J. C. *, Donghia, N., Ferrante, T. C., Dy, A. J., Hsu, K. J., Dubner, R. S., Pardee, K., Jewett, M. C. †, & Collins, J. J. †
Science Advances (2018) doi: 10.1126/sciadv.aat5105
Featured in Science, NPR, Northwestern Engineering Magazine, Northwestern University News, MIT News, Wyss Institute News, Addgene, The Science Breaker, Army Research News, National Science Foundation News
Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
Jaroentomeechai, T. *, Stark, J. C. *, Natarajan, A., Glasscock, C., Mrksich, M., Jewett, M. C. †, & DeLisa, M. P. †
Nature Communications (2018) doi: 10.1038/s41467-018-05110-x
Featured in Chemical & Engineering News (July 2018 & Nov 2018), Northwestern University News & Newsletter, Cornell University News
A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases
Schoborg, J. A. *, Hershewe, J. M. *, Stark, J. C., Kightlinger, W. K., Kath, J. E., Jaroentomeechai, T., Natarajan, A., DeLisa, M. P., & Jewett, M. C. †
Biotechnology & Bioengineering (2018) doi: 10.1002/bit.26502
A pipeline for studying and engineering single-subunit oligosaccharyltransferases
Jaroentomeechai, T., Zheng, X., Hershewe, J. M., Stark, J. C., Jewett, M. C., & DeLisa, M. P. †
Methods in Enzymology (2017) doi:10.1016/bs.mie.2017.07.011
Cell-free synthetic biology: Engineering beyond the cell
Perez, J. G. *, Stark, J. C. *, & Jewett, M. C. †
Cold Spring Harbor Perspectives in Biology (2016) doi: 10.1101/cshperspect.a023853
Energizing eukaryotic cell-free protein synthesis with glucose metabolism
Anderson, M. J. *, Stark, J. C. *, Hodgman, C. E., & Jewett, M. C. †
FEBS Letters (2015) doi: 10.1016/j.febslet.2015.05.045
Repurposing a bacterial quality control mechanism to enhance enzyme production in living cells
Boock, J. T., King, B. C., Taw, M., Conrado, R. J., Siu, K.-H., Stark, J. C., Walker, L. P., Gibson, D. M., & DeLisa, M. P. †
Journal of Molecular Biology (2015) doi: 10.1016/j.jmb.2015.01.003