Publications
( * equal contribution; † corresponding author; Stark Lab personnel in bold )
Antibody-lectin chimeras for glyco-immune checkpoint blockade
Stark JC †, Gray MA, Ibarlucea-Benitez I, Lustig M, Bond A, Cho B *, Govil I *, Luu T *, Priestley MJ *, Veth TS, Errington WJ, Bruncsics B, Ribi MK, Williams LA, Sarkar CA, Wisnovsky S, Riley NM, Morrissey MA, Valerius T, Ravetch JV, & Bertozzi CR †
Nature Biotechnology (2025) doi: 10.1038/s41587-025-02884-6 [open access, bioRxiv 2024]
Featured in Nature Biotechnology, MIT News
Sialylated glycoproteins suppress immune cell killing by binding to Siglec-7 and Siglec-9 in prostate cancer
Wen RM, Stark JC, Marti GEW, Fan Z, Lyu A, Garcia Marques FJ, Zhang X, Riley NM, Totten SM, Bermudez A, Nolley R, Zhao H, Fong L, Engleman EG, Pitteri SJ, Bertozzi CR, & Brooks JD †
Journal of Clinical Investigation (2024) doi.org/10.1172/JCI180282
The glyco-immune checkpoint receptor Siglec-7 interacts with T-cell ligands and regulates T-cell activation
Stewart N, Daly J, Drummond-Guy O, Krishnamoorthy V, Stark JC, Riley NM, Williams K, Bertozzi CR, & Wisnovsky S †
Journal of Biological Chemistry (2024) doi.org/10.1016/j.jbc.2023.105579
Rapid biosynthesis of glycoprotein therapeutics and vaccines from freeze-dried bacterial cell lysates
Stark, JC †, Jaroentomeechai T, Warfel KF, Hershewe JM, DeLisa MP †, & Jewett MC †
Nature Protocols (2023) doi.org/10.1038/s41596-022-00799-z
MYC driven synthesis of Siglec ligands is a novel glyco-immune checkpoint
Smith BAH *, Deutzmann A *, Correa KM, Delaveris CS, Dhanasekaran R, Dove CG, Sullivan DK, Wisnovsky S, Stark JC, Pluvinage JV, Swaminathan S, Riley NM, Rajan A, Majeti R, Felsher DW †, & Bertozzi CR †
Proceedings of the National Academy of Sciences USA (2023) doi.org/10.1073/pnas.2215376120
Genome-wide, bidirectional CRISPR screens identify mucins as critical host factors modulating SARS-CoV-2 infection
Biering SB *, Sarnik SA *, Wang E, Zengel JR, Leist SR, Schäfer A, Sathyan V, Hawkins P, Okuda K, Tau C, Jangid AR, Duffy CR, Wei J, Gilmore RC, Alfajaro MM, Strine MS, Nguyenla X, Van Dis E, Catamura C, Yamashiro LH, Belk JA, Begeman A, Stark JC, Shon DJ, Fox DM, Ezzatpour S, Huang E, Olegario N, Rustagi A, Volmer AS, Livraghi-Butrico A, Wehri E, Behringer RB, Cheon D, Schaletzky J, Aguilar HC, Puschnik AS, Button B, Pinsky BA, Blish CA, Baric RS, O’Neal WK, Bertozzi CR, Wilen CB, Boucher RC, Carette JE, Stanley SA, Harris E †, Konermann S †, & Hsu PD †
Nature Genetics (2022) doi: 10.1038/s41588-022-01131-x [bioRxiv 2021]
Targeting hypersialylation in multiple myeloma represents a novel therapeutic strategy to enhance NK cell-mediated tumor responses
Daly J, Sarkar S, Natoni A, Stark JC, Riley NM, Bertozzi CR, Carlsten M †, O’Dwyer M †
Blood Advances (2022) doi: 10.1182/bloodadvances.2021006805
Implementing hands-on molecular and synthetic biology education using cell-free technology
Huang A †, Bryan B, Kraves S, Alvarez-Saavedra E, Stark JC †
Methods in Molecular Biology (2022) doi: 10.1007/978-1-1998-8_25
Synthetic Siglec-9 agonists inhibit neutrophil activation associated with COVID-19
Delaveris CS, Wilk AJ, Riley NM, Stark JC, Yang SS, Rogers AJ, Ranganath T, Nadeau KC, The Stanford COVID-19 Biobank, Blish CA, & Bertozzi CR †
ACS Central Science (2021) doi: 10.1021/acscentsci.0c01669 [open access]
On-demand biomanufacturing of protective conjugate vaccines
Stark JC *, Jaroentomeechai T *, Moeller TD, Hershewe JM, Warfel KF, Moricz BS, Martini AM, Dubner RS, Hsu KJ, Stevenson TC, Jones BD, DeLisa MP †, & Jewett MC †
Science Advances (2021) doi: 10.1126/sciadv.abe9444 [open access, bioRxiv 2019]
Featured in Nature, PNAS, preLights, Vice, Northwestern University News, Cornell University News
A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
Kightlinger W, Duncker KE, Ramesh A, Thames AH, Natarajan A, Stark JC, Yang A, Lin L, Mrksich M, DeLisa MP, & Jewett MC †
Nature Communications (2019) doi: 10.1038/s41467-019-12024-9 [open access, bioRxiv 2019]
Featured in Northwestern Engineering News
BioBits™ Health: Classroom activities exploring engineering, biology, and human health with fluorescent readouts
Stark JC, Huang A, Hsu KJ, Dubner RS, Forbrook J, Marshalla S, Rodriguez F, Washington M, Rybnicky GA, Nguyen PQ, Hasselbacher B, Jabri R, Kamran R, Koralewski V, Wightkin W, Martinez T, & Jewett MC †
ACS Synthetic Biology (2019) doi: 10.1021/acssynbio.8b00381 [open access]
Featured in Northwestern University News, National Science Foundation News & Blog
BioBits™ Bright: A fluorescent synthetic biology education kit
Stark JC *, Huang A *, Nguyen PQ *, Dubner RS, Hsu KJ, Ferrante TC, Anderson MA, Kanapskyte A, Mucha Q, Packett JS, Patel P, Patel R, Qaq D, Zondor T, Burke J, Martinez T, Miller-Berry A, Puppala A, Reichert K, Schmid M, Brand L, Chellaswamy JF, Faheem N, Fetherling S, Gong E, Gonzalzles EM, Granito T, Koritsaris J, Nguyen B, Ottman S, Palffy C, Patel A, Skweres S, Slaton A, Woods T, Donghia N, Pardee K, Collins JJ †, & Jewett MC †
Science Advances (2018) doi: 10.1126/sciadv.aat5107 [open access]
Featured in Science, NPR, Northwestern Engineering Magazine, Northwestern University News, MIT News, Wyss Institute News, Addgene, The Science Breaker, Army Research News, National Science Foundation News
BioBits™ Explorer: A modular synthetic biology education kit
Huang A *, Nguyen PQ *, Stark JC *, Donghia N, Ferrante TC, Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC †, & Collins JJ †
Science Advances (2018) doi: 10.1126/sciadv.aat5105 [open access]
Featured in Science, NPR, Northwestern Engineering Magazine, Northwestern University News, MIT News, Wyss Institute News, Addgene, The Science Breaker, Army Research News, National Science Foundation News
Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
Jaroentomeechai T *, Stark JC *, Natarajan A, Glasscock C, Mrksich M, Jewett MC †, & DeLisa MP †
Nature Communications (2018) doi: 10.1038/s41467-018-05110-x [open access]
Featured in Chemical & Engineering News (July 2018 & Nov 2018), Northwestern University News & Newsletter, Cornell University News
A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases
Schoborg JA *, Hershewe JM *, Stark JC, Kightlinger WK, Kath JE, Jaroentomeechai T, Natarajan A, DeLisa MP, & Jewett MC †
Biotechnology & Bioengineering (2018) doi: 10.1002/bit.26502 [bioRxiv 2017]
A pipeline for studying and engineering single-subunit oligosaccharyltransferases
Jaroentomeechai T, Zheng X, Hershewe JM, Stark JC, Jewett MC, & DeLisa MP †
Methods in Enzymology (2017) doi:10.1016/bs.mie.2017.07.011 [bioRxiv 2017]
Cell-free synthetic biology: Engineering beyond the cell
Perez JG *, Stark JC *, & Jewett MC †
Cold Spring Harbor Perspectives in Biology (2016) doi: 10.1101/cshperspect.a023853
Energizing eukaryotic cell-free protein synthesis with glucose metabolism
Anderson MJ *, Stark JC *, Hodgman CE, & Jewett MC †
FEBS Letters (2015) doi: 10.1016/j.febslet.2015.05.045
Repurposing a bacterial quality control mechanism to enhance enzyme production in living cells
Boock JT, King BC, Taw M, Conrado RJ, Siu, K-H, Stark JC, Walker LP, Gibson DM, & DeLisa MP †
Journal of Molecular Biology (2015) doi: 10.1016/j.jmb.2015.01.003